ApoTome introduces optical sectioning capabilities to your widefield imaging system simply by inserting the ApoTome slider into the field stop position. Unsing structured illumination, out og focus blur is efficiently removed, resulting in brilliant optical sectioning of highest contrast and optimal resolution
The ApoTome system uses a technique called structured illumination to project a grid into the focal plane of a widefield fluorescent microscope. The grid is precisely moved and several images are acquired at each focus plane, each with the grid in a slightly different location.
Single parts of the samle in focus vary more significantly than those out of focus, it is possible to automatically generate an optical section free of out of focus blur.
Whilst the time to acquire is a little slower than a deconvolution only approach this technique offers greater levels of out of focus discrimination.Applications:
- Neuronal branching in brain sections captured in brilliant multicolor images
- Studying tissue formation in embryo development with low bleaching
- Capture subcelluar structures in three dimensions with an easy-to-use setup and short acquisition times
- Analyze fluorescent markers in pathological sections without out of focus blur