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Spinning Disk

Cell Observer SD combineds the speed, modularity, and reability of the Zeiss Cell Observer with the fast confocal image acquisition of the Yokagava CSU-X in a seamlessly integrated modular system for fast confocal live cell imaging
Cell Observer SD




Spinning disk microskopes project hundreds of circular apertures simultaneously into the sample and collect the returning emission through the same apertures. The beams are not actually scanned but produced by spinning the disk rapidly to illuminate the whole field of view. Out of focus light is almost entirely rejected by the disk, meaning the acquired image is already optically sectioned without the need for software processing.

Spinning disks systems ara a form of parallel confocal, meaning they work on multiple areas of the sample at the same time to trade a little resolution for a lot of speed.

Applications:

  • Image subcelluar trafficking in 3D over time with maximum acquisition speed
  • Visualize cytoskeletal dynamics with highest sensitivity
  • Functional imaging of cellular signal transduction with high temporal resolution
  • Long-term confocal live cell imaging with lowest phototoxicity