Cell Observer
Modern life science research calls for a powerful imaging system that can be used flexibly, is easy to operate, and is equipped with a whole host of functionalites. A convincing example of such af system is Cell Observer from Carl Zeiss
Cell Observer and Deconvolution
Widefield fluorescence microscopes acquire both the in-focus and out of focus contributions from the sample. This makes them one of the most sensitive systems for live cell imaging because no emitted light is thrown away. The majority of the out of focus light is derived from the point spread function (PSF). A precise model for the PSF can be used to devonvolve image stacks. It is both a very efficient and cost-effective approch to generate 3D data.
Deconvolution can also be used to enhance other optical sectioning techniques.
Applications:
- Live cell imaging with the whole range of available fluorescent proteins with extreme sensitivity
- Quantitative imaging of intracellular trafficking events at high speed
- Perform simultaneous quantitative ion or FRET imaging with dual camera acquistion
- Capture morphology changes in high resolution using deconvolution microscopy
- Quantify cell cycle studies with long-term time-lapse microscopy
