LSM 710 NLO - LSM 7 MP
LSM 710 NLO and LSM 7 MP are perfect for sensitive in-depth analysis of live specimens, including whole organismus. Both systems are outstanding in their unmatched sensitivity: Precise settings of the femtosecond laser and most effective non-descanned detection provide efficient excitation and imaging deep within tissue. Bleaching and manipulation experiments will succeed with pinpoint accuracy due to 3D-defined exitation
Multiphoton
Multiphoton microscopes (MPMs), als commonly called Two Photon (2P) or Non Linear Optics (NLO), also rapidly scan a single beam across the sample and are a form of LSM. Multiphoton microscopes utilize a pulsed infrared laser to focus light into the sample.
In the focal plane the photon density reaches a point where two or more photons can excite a fluorophore in a similar way to a single photon with half the wavelength. The pulsed light is too weak outside of the focal plane to create emission, and so no pinhole is necessary to reject out of focus light.
Because there is no need for a pinhole, NLO systems are technically not confocal.
Applications:
- Developmental studies over long time periods with minimized damage to the sample
- Combine electrophysiology and imaging to get best images from patched cells within a tissue slice or animal
- Precise results from uncaging experiments due to excactly defined uncaged volumes
